Preparation, identification, and activity evaluation of antioxidant peptides from protein hydrolysate of corn germ meal

Published: 20 August 2019

Author: Sitian Zhang, Mingdi Zhang, Ruiwen Yang, Simin Zhang, Songyi Lin            

Journal: Food Processing and Preservation   

Abstract

Information on antioxidant peptides derived from corn germ meal (CGM) is seldom reported. In this paper, three novel antioxidant peptides including Met–Gly–Gly–Asn (MGGN; 377.42 Da), Met–Asn–Asn (MNN; 377.42 Da), and Met–Glu–Asn (MEN; 392.43 Da) were purified from the most active fractions of corn germ meal hydrolysate, and their activity was evaluated by radical scavenging, oxygen radical absorbance capacity, cellular antioxidant activity (CAA), and intracellular reactive oxygen species scavenging assays. MNN had a higher value for the CAA assay (1,213.79 μmol of QE/100 mmol) and could be most effective in preventing conditions favorable to intracellular oxidative stress. Finally, electron paramagnetic resonance spectroscopy directly verified the above experimental results by measuring the number of •OH trapped in the system. Thus, CGM could be considered as a potential source of antioxidant peptide and MNN might be regarded as a natural antioxidant.   Go to Article Link